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Add my name to your mailing list. (Please take a moment to answer 5 quick
questions about fluorescence in your application.)
1) What fluorescence detection methods do you currently use?
Fluorescene Microscope a) w/ CCD camera
Spectrofluorometer
Microplate Fluorometer a) Polarization b) Time-Resolved
Tube Fluorometer a) Polarization
Flow Cytometer
Sequencer-Fluorescent a) UV b) Vis c) NIR
Other
2) What fluorescent labelled probes are you using?
Cytokines Haptens Peptides
Mononucleotides Oligonucleotides
Monoclonal antibodies
Polyclonal antibodies:
Anti-Human Anti-Mouse Anti-Rabbit Other
Other
3) What fluorophores are you using in your lab now?
Fluorescein Rhodamine
Cynanines (emission < 700) Cynanines (emission > 700)
Other
4) Please rank the following attributes of a flurophore 1-7, with 1 being most
important to you and 7 begin least important:
PhotoStability Solubility
Decay Time Ease of Labelling
Available as Labelling Kit Stoke Shift
Extinction Coefficient
Other
5) What are your primary applications for fluorescence?
DNA Sequencing FISH
Flow Cytometry Cell Imaging
Gel Imaging Drug discovery
Screening Assay, Clinical
Assay, Veterinary Assay, Environmental
Biosensor Ab: Protein Studies
Comments/Questions?
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